Temperature affects all processes, including development, and yet, it remains unknown how and if underlying mechanical and biochemical events are coordinated to slow down development robustly at lower temperatures. The zebrafish is an ideal organism in which temperature's effect on development can be probed at the cellular level across a wide temperature range. Using zebrafish embryos, we have studied the vulnerabilities of epithelium during gastrulation at sub-optimal temperatures (20\degree C). The enveloping layer (EVL) in the cold displays large multi-nucleated cells stemming from cytokinesis failures. Differences in tissue tension during late epiboly also leads to rupturing of the EVL in certain embryos. Furthermore, mesendoderm patterning reveals convergence-extension defects under varying temperatures, suggesting that different aspects of gastrulation are affected in parallel. In this talk I will dicuss our work focusing on the effect of temperature on zebrafish gastrulation, thereby elucidating why development fails under sub-optimal temperatures.
Zoom Link: https://icts-res-in.zoom.us/j/98354053917?pwd=wfFl3iE2Zar4yCHgOaNgPzqpfIldG7.1
Meeting ID: 983 5405 3917
Passcode: 202030